The deceased were identified as Gonesh Shonnayashi and Kolpona Shonnayashi.
At least 25 passengers were also injured in the accident.
Highway police and Fire Service sources said Gonesh and his wife, residents of Shibalaya upazila, died when an Aricha-bound bus from Manikganj fell into a roadside ditch at Tora area of Ghior upazila on Dhaka-Manikganj highway around 6pm.
The bus was giving space to a private car coming from the opposite direction.
Arifur Rahman, Sub-Inspector of Borongol Highway police outpost, said on information the police and fire service conducted rescue operation with the help of the locals.
The injured were taken to Manikganj district hospital but the couple succumbed to their injuries on the way.
The other injured are being treated at the hospital.