He came up with the allegation while addressing the 75th session of the UN General Assembly, reports Xinhua.
"We're in economic war through US sanctions on Iranian financial, economic and business institutions,” he told the UNGA.
Iran is grappling with "the harshest sanctions in history imposed in blatant and gross violation of the Charter of the United Nations and international agreements", Rouhani said.
Also read: Iran sues Trump for killing Gen Soleimani
US President Donald Trump withdrew from the 2015 landmark Iranian nuclear deal, internationally known as the Joint Comprehensive Plan of Action (JCPOA), in May 2018 and unilaterally re-imposed financial and economic sanctions against Iran.