The deceased was identified as Rafi, 45, wife of Moti of Gopalganj district.
Dr Shoilendranath Biswas, resident doctor of KMCH, said Rafi got admitted to the hospital around 2pm with dengue fever and died around 11am.
Nineteen people have reportedly died of dengue in Khulna this year.
Meanwhile, 267 people were hospitalised with the mosquito-borne disease in 24 hours till Tuesday morning. Currently, 1,316 dengue patients are being treated at different hospitals.
The government said 90,545 people were hospitalised with dengue across the country since the beginning of the year. Of them, 88,987 recovered fully.
The Institute of Epidemiology, Disease Control and Research (IEDCR) confirmed the deaths of 81 dengue patients after reviewing 136 reports out of 242 cases.